Evaluation of rapid methods of identifying group B streptococci.

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Evaluation of rapid methods of identifying group B streptococci.

Rapid methods of identifying Lancefield group B streptococci were compared to the standard Fuller's extraction method. Such tests as sodium hippurate hydrolysis, bile tolerance, aesculin hydrolysis, pyruvate fermentation, Camp factor, pigmentation, and bacitracin haemolysis were tested on both routine clinical specimens and National Collection of Type Culture strains. The results show that pigm...

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Rapid identification of group B streptococci by counterimmunoelectrophoresis.

Beta-hemolytic streptococcal isolates have been examined by counterimmunoelectrophoresis (CIE) with group B antiserum to determine whether this techinque is of value in the rapid identification of group B strains. Ninety stock cultures and 100 clinical isolates of beta-hemolytic streptococci including representatives of groups A, D, C, G, and B were inoculated into Todd-Hewitt broth; after incu...

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Rapid slide coagglutination test for identifying and typing group B streptococci.

A Cowan I strain of Staphylococcus aureus was labeled with either group B streptococcal grouping or typing antiserum. These antibody-labeled reagent cells (ARC) were used in a slide coagglutination test to identify and type group B streptococci from blood agar plates. All streptococci were also identified by the standard Lancefield capillary precipitin test. In a blind study, all 141 group B st...

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Rapid detection of group B streptococci directly from vaginal swabs.

Duplicate vaginal swabs were obtained from patients who attended obstetric or gynecologic clinics affiliated with the Magee Womens Hospital in Pittsburgh. One swab was cultured semiquantitatively on 5% sheep blood agar to detect group B streptococci (GBS). The other swab was subjected to a rapid method (25 min) for antigen detection and micronitrous acid exposure to extract the GBS antigen, fol...

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Pharyngeal carriage of group B streptococci: detection by three methods.

The prevalence of pharyngeal carriage of group B streptococci was evaluated in patients with and without the complaint of a sore throat by three methods (blood agar plates, Columbia CNA agar plates, and a selective enrichment broth containing gentamicin and nalidixic acid). The overall carriage rate of group B streptococci was 12%, and there was no significant difference between the two groups ...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1980

ISSN: 0021-9746

DOI: 10.1136/jcp.33.3.302